Not known Factual Statements About flow cytometer
Not known Factual Statements About flow cytometer
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Data-enriched characterization of cell populations. Morphological data from visuals can insert towards the richness of flow cytometry info. As an example, the determine exhibits an if not traditional apoptosis assay working with Annexin V and PI, incorporating cell imaging to characterize cells in each inhabitants to reveal morphologically unique characteristics. These insights couldn't happen to be gained from multiplex staining by yourself.
As samples are obtained around the Attune CytPix Flow Cytometer, the higher-velocity brightfield camera captures and merchants pictures of detected occasions at a amount up to 6,000 photographs for each second, according to the flow price and impression size. For greater adaptability, Attune Cytometric Program allows you to adjust the image capture frequency as necessary and select a selected gate for image seize, if required.
(B) RAW 264.7 macrophages are sometimes annotated inaccurately with respect to particle count due to their complexity and noncircular borders. The amount of singlets obtainable for Evaluation increases as soon as the consumer trains and employs a more accurate product.
Find out new insights into your experimental info by visualizing populations employing merged fluorescence and morphology details. Our software interface wizards enable downsampling, concatenation (merged files) and dimensionality instruments that happen to be built into your application for an intuitive, streamlined details Investigation knowledge.
"An image is not pretty worth one thousand dots, but it really seriously really helps to see visuals. It is excellent to check out pictures that correlate with staining. Such as, AnnexinV-constructive cells which are morphologically different than wholesome cells, or CD14+ monocytes which might be larger and much more textured than CD3+/CD4+/CD14- lymphocytes.
In this article, a seasoned person has gated singlets confidently. After analyzing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate incorporates a lot more than 4% aggregates.
Pair your Attune flow cytometer having an autosampler for improved workflow performance, and insert robotic integration for maximum throughput.
Reliable impression high quality even at large throughput Acoustic focusing plus a significant-pace camera Mix to graphic these T cells continually at very low or high flow charges. Quickly alter concentration and camera configurations to meet experimental requirements.
The Attune NxT cytometer is definitely the trusted workhorse instrument your lab can count on for reliable and trustworthy functionality. The Attune CytPix design incorporates a superior-velocity brightfield digital camera and AI-pushed image analysis application, with modern upgrades including equipment learning resources and an intuitive consumer interface, enabling enhanced facts precision, precision, and more rapidly insights into sample biology.
People can look at celebration visuals in serious-time to confirm sample quality, enhance protocols, and document the morphology of every cell population identified for Investigation. Imaging capture also benefits from acoustic concentrating, which really helps to position the cells to ensure a sharp, centered impression is obtained.
Audio waves help specific cell alignment, leading to Outstanding facts high-quality and ~10X speedier sample flow charges.
We Earlier shown the strength of imaging Vehicle-T/Ramos cell interactions. Enable’s take a look at just the population of finest fascination, the double favourable gatherings, to learn more. We are able to now use extended morphological parameters (listed here, circularity and skewness of depth) to even more study the capabilities of these populations and refine gating on these activities, increasing details robustness.
Created-in dimensionality reduction instruments amplified resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to confirm envisioned population resolution in lysed whole human blood. Consumer can look at situations from Each and every cluster for Visible confirmation of cell phenotypes.
Adaptable payment program from Agilent Financial Options help you get the vital instrumentation you require for immediate undertaking, or to improve your lab.
Gating on these extended parameters lets you quickly and correctly detect populations of interest and confirm gating system with little or no manual evaluate. The Attune software program gives entry to state-of-the-artwork device Mastering analysis equipment within just an expertly designed user interface, rendering it extremely user friendly.
Because the mobile line experienced Earlier been analyzed around the Attune CytPix Flow Cytometer, the scientists went again to the photographs and were being capable of document the microbial infection at the least five times before.
The Attune CytPix Flow Cytometer permits customers to visualize samples for the duration of acquisition, verify the precision of gating approaches, and help final here results with responsible documentation for publication purposes.
What appeared to be a homogeneous inhabitants is definitely more numerous—an insight that should be viewed as when interpreting effects.